pH Gradient flattening in isoelectric fo
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Ray N. Rapaport; Andrew Jackiw; Ray K. Brown
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Article
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1980
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John Wiley and Sons
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English
β 458 KB
## Abstract Isoelectric focusing in 60 cm long gels improves resolution in multipleβcomponent systems by providing very shallow, linear pH gradients. Broad range ampholytes available commercially or synthesized in our laboratory develop pH gradients of 0.1 pH/cm at voltages of 1500 to 4500 V, with