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Protein-load effects on the pH gradient of isoelectric focusing in polyacrylamide gels

✍ Scribed by Sarah J. Cantrell; Joseph A. Babitch; Susanna Torres


Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
976 KB
Volume
116
Category
Article
ISSN
0003-2697

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pH Gradient flattening in isoelectric fo
✍ Ray N. Rapaport; Andrew Jackiw; Ray K. Brown πŸ“‚ Article πŸ“… 1980 πŸ› John Wiley and Sons 🌐 English βš– 458 KB

## Abstract Isoelectric focusing in 60 cm long gels improves resolution in multiple‐component systems by providing very shallow, linear pH gradients. Broad range ampholytes available commercially or synthesized in our laboratory develop pH gradients of 0.1 pH/cm at voltages of 1500 to 4500 V, with