Effect of salt on the performance of immobilized pH gradient isoelectric focusing gels
✍ Scribed by John R. Strahler; Samir M. Hanash; Luke Somerlot; Bengt Bjellqvist; Angelika Görg
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 820 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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ture antibody, B140 (5 l), was added to the resulting supernatant fraction (1 ml) and the A content was analyzed using the method described above. The ratio of A 42/A 40 in the RIPA-soluble fraction was 0.073, which was similar to the ratio calculated directly (A 40, 15.48 g/ml; A 42, 1.08 g/ml
## Abstract Isoelectric focusing on immobilized pH gradients dissociates most complexes between fatty acids and serum albumin from whole plasma. Most albumin thus migrates to a pI of 5.8, very close to the theoretical value of 6.0 as calculated with the Linderstrøm‐Lang equation. The phenomenon was