The behavior of serum albumin upon isoelectric focusing on immobilized pH gradients
β Scribed by Dr. Elisabetta Gianazza; Adele Frigerio; Silvia Astrua-Testori; Pier Giorgio Righetti
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 322 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0173-0835
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β¦ Synopsis
Abstract
Isoelectric focusing on immobilized pH gradients dissociates most complexes between fatty acids and serum albumin from whole plasma. Most albumin thus migrates to a pI of 5.8, very close to the theoretical value of 6.0 as calculated with the LinderstrΓΈmβLang equation. The phenomenon was elucidated through saturation experiments and timeβcourse monitoring of the protein pattern upon focusing.
π SIMILAR VOLUMES
## Isoelectric focusing in immobilized pH gradients of phosphoglucomutase and esterases from the spiny lobster A method is described for detecting polymorphisms ofcephalothorax and tail homogenates of 25 puerulus staged Panulirus argus in phosphoglucomutase (PGM) and esterases. Isoelectric focusin