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The behavior of serum albumin upon isoelectric focusing on immobilized pH gradients

✍ Scribed by Dr. Elisabetta Gianazza; Adele Frigerio; Silvia Astrua-Testori; Pier Giorgio Righetti


Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
322 KB
Volume
5
Category
Article
ISSN
0173-0835

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✦ Synopsis


Abstract

Isoelectric focusing on immobilized pH gradients dissociates most complexes between fatty acids and serum albumin from whole plasma. Most albumin thus migrates to a pI of 5.8, very close to the theoretical value of 6.0 as calculated with the LinderstrΓΈm‐Lang equation. The phenomenon was elucidated through saturation experiments and time‐course monitoring of the protein pattern upon focusing.


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