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Isoelectric focusing in immobilized pH gradients of phosphoglucomutase and esterases from the spiny lobster

✍ Scribed by Georgina Espinosa; Dr. Elisabeth Wenisch; Herbert Danner; Hermann Katinger; Pier Giorgio Righetti

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
385 KB
Volume
11
Category
Article
ISSN
0173-0835

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✦ Synopsis

Isoelectric focusing in immobilized pH gradients of phosphoglucomutase and esterases from the spiny lobster

A method is described for detecting polymorphisms ofcephalothorax and tail homogenates of 25 puerulus staged Panulirus argus in phosphoglucomutase (PGM) and esterases. Isoelectric focusing in immobilized pH gradients was used. In the pH 6.0-8.0 interval for phosphoglucomutase and in the pH 3.5-5.0 and 4.2-4.9 ranges for esterases, both enzymes appeared as polymorphic band patterns. These could be explained by one locus with 2 alleles for phosphoglucomutase and 3 loci with 2,3 and 4 alleles for esterases. Esterases exhibit a more extensive polymorphism in immobilized pH gradients than in polyacrylamide gel electrophoresis.

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✍ Dr. Elisabetta Gianazza; Adele Frigerio; Silvia Astrua-Testori; Pier Giorgio Rig πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 322 KB πŸ‘ 1 views

## Abstract Isoelectric focusing on immobilized pH gradients dissociates most complexes between fatty acids and serum albumin from whole plasma. Most albumin thus migrates to a pI of 5.8, very close to the theoretical value of 6.0 as calculated with the LinderstrΓΈm‐Lang equation. The phenomenon was