A new applicator strip with a concentration effect for isoelectric focusing
✍ Scribed by Dr. Werner Pflug; Bela Laczko; Fritz Müller
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 200 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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## Abstract Proteins were separated by capillary isoelectric focusing (CIEF) performed in a 5‐μm i.d., 12‐cm long uncoated capillary, and detected by an on‐column concentration gradient detector based on Schlieren optics. The narrow capillary allows the application of a 15‐kV separation voltage. Th
A rapid and reproducible method for the pH measurementin the effluent from density gradient electrofocusing is described. By this procedure, after preparative isoelectric focusing, the detection of protein zones and pH measurement can be accomplished simultaneously, by serially coupling a uv flow ce
## Abstract Capillary isoelectric focusing (CIEF) separations are usually performed with neutral coated fused‐silica capillaries in aqueous anticonvective media. Glycerol, a very viscous solvent (η = 945 mPa·s at 25°C), known to help stabilize any kind of proteins and solubilize hydrophobic ones, w