✦ LIBER ✦

A new method of cloning mammalian tissue culture cells in suspension

✍ Scribed by R. A. Walters; J. Y. Hutson; B. R. Burchill

Publisher: John Wiley and Sons
Year: 1970
Tongue: English
Weight: 355 KB
Volume: 76
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040760112

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Chinese hamster cells (line CHO) were cloned in suspension with a carboxymethylcellulose‐containing medium, and the colonies were counted with an electronic particle counter after enzymatically degrading the carboxymethylcellulose with cellulase. Survival of x‐irradiated cells was determined by both the standard plating technique and the suspension technique, and comparable results were obtained.

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