𝔖 Bobbio Scriptorium
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Novel anchorage matrices for suspension culture of mammalian cells

✍ Scribed by Z. Bohak; A. Kadouri; M. V. Sussman; A. F. Feldman

Publisher
Wiley (John Wiley & Sons)
Year
1987
Tongue
English
Weight
912 KB
Volume
26
Category
Article
ISSN
0006-3525

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✦ Synopsis

Nonwoven fabrics were used as a support matrix for culturing anchorage-dependent diploid human lung fibroblast (IMR-90) cells. The mast significant advantage of the fabrics is that low-inocula concentrations suffice to attain high final cell density. Cultures were successfully grown from inocula containing as little as 5 8 of the final number of cells, which is significantly lower than the 30-408 inocula concentrations typically required for tissue cell culture on dextran bead microcarriers, or on petri dishes or culture flasks. Nonwoven fabric cell supports also were superior to conventional spherical microcarriers for production of metabolic biopolymers (t-plasminogen activator) in serum-free media.

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