✦ LIBER ✦

A Quantitative Method for cDNA-Directed Expression in Cultured Mammalian Cells

✍ Scribed by T. Aoyama; T. Nakajimanasu; M. Souri; T. Hashimoto

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 471 KB
Volume: 227
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1995.1256

No coin nor oath required. For personal study only.

✦ Synopsis

A simple technique of quantitative cDNA-directed expression was devised by using a vaccinia virus system. The technique was applied to the cDNA expression of peroxisomal acyl-coenzyme (A) oxidase ( (A O X) ) and microsomal cytochromes P450 1 A2, 2E 1, and 3A4. Quantitative cDNA expression of all four enzymes tested was successfully achieved. This represents important progress for establishing the relationship between the amount of enzyme and its function in cells. The vaccinia virus system was also used to express AOX protein in specific cellular regions. 1995 Academic Press, Inc.

📜 SIMILAR VOLUMES

A new method of cloning mammalian tissue

A new method of cloning mammalian tissue culture cells in suspension

✍ R. A. Walters; J. Y. Hutson; B. R. Burchill 📂 Article 📅 1970 🏛 John Wiley and Sons 🌐 English ⚖ 355 KB

## Abstract Chinese hamster cells (line CHO) were cloned in suspension with a carboxymethylcellulose‐containing medium, and the colonies were counted with an electronic particle counter after enzymatically degrading the carboxymethylcellulose with cellulase. Survival of x‐irradiated cells was deter

A quantitative comparison of the mutagen

A quantitative comparison of the mutagenicity of carcinogenic polycyclic hydrocarbon derivatives in cultured mammalian cells

✍ R. F. Newbold; P. Brookes; R. G. Harvey 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 657 KB

## Abstract The mutagenicity of a series of reactive polycyclic hydrocarbon derivatives has been studied using Chinese hamster (V79) cells in culture and, as a mutational marker, resistance to the purine analogue 8‐azaguanine. The compounds were compared by relating mutation frequency to the dose a

A Spectrophotometric Method for the Dire

A Spectrophotometric Method for the Direct Detection and Quantitation of Nitric Oxide, Nitrite, and Nitrate in Cell Culture Media

✍ Lisa A. Ridnour; Julia E. Sim; Michael A. Hayward; David A. Wink; Sean M. Martin 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 117 KB

A method for the spectrophotometric determination of nitric oxide, nitrite, and nitrate in tissue culture media is presented. The method is based on the nitric oxide-mediated nitrosative modification of sulfanilic acid that reacts with N-(1-naphthyl)ethylenediamine dihydrochloride forming an orange-

Large-scale production of endotoxin-free

Large-scale production of endotoxin-free plasmids for transient expression in mammalian cell culture

✍ Aleksei Rozkov; Bert Larsson; Stefan Gillström; Robert Björnestedt; Stefan R. Sc 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 219 KB 👁 2 views

## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p

Valproic acid: A viable alternative to s

Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

✍ Gaurav Backliwal; Markus Hildinger; Ivan Kuettel; Fanny Delegrange; David L. Hac 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 198 KB

## Abstract Various DNA methyl transferase inhibitors (iDNMTs) and histone deacetylase inhibitors (iHDACs) were screened for their ability to enhance transient gene expression (TGE) in Human Embryonic Kidney 293‐EBNA (HEK293E) cells. The effects in HEK293E cells were compared to those in Chinese Ha

A novel technique for quantitative detec

A novel technique for quantitative detection of mRNA expression in human bone derived cells cultured on biomaterials

✍ Zreiqat, Hala ;Markovic, Boban ;Walsh, William R. ;Howlett, C. Rolfe 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 674 KB

A nonisotopic and quantitative in situ hybridization technique was adapted to investigate the effect of biomaterials on the cellular expression of mRNA from human bone derived cells (HBD cells). HBD cells were cultured for 24 or 48 h on tissue culture plastic, alumina, and ion modified alumina. Oste