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Five-year perspective of the large-scale growth of mammalian cells in suspension culture

✍ Scribed by R. K. Zwerner; R. M. Cox; J. D. Lynn; R. T. Acton


Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
919 KB
Volume
23
Category
Article
ISSN
0006-3592

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## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p