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A new colorimetric determination of d-amino acid oxidase and urate oxidase activity

✍ Scribed by Takafumi Watanabe; Yasuo Motomura; Tetsuya Suga

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 305 KB
Volume: 86
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90347-0

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✦ Synopsis

A new method of calorimetric determination of n-amino acid oxidase and mate oxidase using catalase and 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole is reported. This method is based on the combination oftwo steps ofenzyme reactions and calorimetric procedure. The values obtained by this method are satisfactorily correlated with those obtained by the dinitrophenylhydrazine method for o-amino acid oxidase activity and the ultraviolet method for urate oxidase activity and showed good reproducibility and accuracy. It is considered that the method can be useful as a method of activity determination for studying enzyme kinetics and the reaction mechanism.

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