A modification of the adriamycin quantitative assay for DNA is presented which is sensitive to I-20 pg DNA. The assay is simple, rapid, and highly reproducible but, contrary to previous claims, not specific for DNA. RNA interferes with the assay while protein does not. The mechanism and sensitivity
A modified spectrofluorometric assay for DNA using adriamycin
โ Scribed by D.G. Kennedy; J. Nelson; H.W. van den Berg; R.F. Murphy
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 266 KB
- Volume
- 167
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A spectrofluorometric assay for DNA is described. The changes in the fluorescence of adriamycin when it binds to DNA may be quantitated in terms of the amount of DNA present. The assay is specific and reproducible, being free from interference from RNA, protein, and sample turbidity.
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