A simple assay for DNA endonucleases
โ Scribed by E. Peter Geiduschek; Allen Daniels
- Publisher
- Elsevier Science
- Year
- 1965
- Tongue
- English
- Weight
- 231 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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Assays for endonuclease and ligase activities have been devised that make use of the fact that circular phage A DNA is preferentially retained on nitrocellulose filters while linear ADNA is not. The assay is rapid, time dependent, and quantitative and has been used for the purification of restrictio
## Abstract The intactness of DNA is an important part of the normal cellular structure. Any change to the DNA in the form of breaks leads to a change in the integrity, which in turn leads to abnormality in the cellular activity. Many discrepancies have been reported among the various methods of de