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A simplified assay for endonuclease and ligase activities

โœ Scribed by Jakob Reiser; Carolyn M. Bentley; Robert Yuan

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
437 KB
Volume
75
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Assays for endonuclease and ligase activities have been devised that make use of the fact that circular phage A DNA is preferentially retained on nitrocellulose filters while linear ADNA is not. The assay is rapid, time dependent, and quantitative and has been used for the purification of restriction endonucleases and DNA ligases.

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