A simple assay for DNA restriction endonucleases
โ Scribed by Frank M. DeFilippes
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 265 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
As a novel type of regulator molecule for DNA-recognizing proteins, a photo-cross-linked oligonucleotide duplex was designed and synthesized. The molecule regulated the activity of a restriction endonuclease by being recognized as a substrate. This type of regulating molecule is regarded as a decoy-
Assays for endonuclease and ligase activities have been devised that make use of the fact that circular phage A DNA is preferentially retained on nitrocellulose filters while linear ADNA is not. The assay is rapid, time dependent, and quantitative and has been used for the purification of restrictio
## Abstract The intactness of DNA is an important part of the normal cellular structure. Any change to the DNA in the form of breaks leads to a change in the integrity, which in turn leads to abnormality in the cellular activity. Many discrepancies have been reported among the various methods of de