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Modification of the quantitative DNA assay using adriamycin

✍ Scribed by David G. Dalbow; Bettie M. Bartuska

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 324 KB
Volume: 95
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90772-3

No coin nor oath required. For personal study only.

✦ Synopsis

A modification of the adriamycin quantitative assay for DNA is presented which is sensitive to I-20 pg DNA. The assay is simple, rapid, and highly reproducible but, contrary to previous claims, not specific for DNA. RNA interferes with the assay while protein does not. The mechanism and sensitivity of the assay is interpreted with reference to published data on adriamycin DNA binding.

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