A modification for “smileless” polyacrylamide gels in nucleic acid sequencing
✍ Scribed by Dr. Donald J. Brown; Timothy J. Bos
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 208 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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A modification of the S. Panyim and R. Chalkley (1%9, Arch. Biochem. Biophys. 130, 337) acetic acid-urea polyacrylamide gel system to include a stacking gel has been devised. The modification makes possible the use of acetic acid-urea gels in the microslab system of P. T. Matsudaira and D. R. Burges
## Fractionation of nucleic acids and their fragments with polyacrylamide gel has been widely applied in sequencing of nucleic acids. Although the conditions of electrophoresis for this purpose have previously been suggested, we have found that polyacrylamide gel electrophoresis at 5000 V (100 V/c
Staining of polyacrylamide gels with methylene blue prior to silver staining increases band resolution and sensitivity. This method permits resolution of multiple bands less than 1 mm apart, and is able to detect bands containing only 100 pg of RNA.