𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A miniaturized electrode configuration for isoelectric focusing

✍ Scribed by Dr. Dirk Megow; Gisela Jacobasch

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
259 KB
Volume
12
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

✦ Synopsis

An electrode configuration is described which allows fast isoelectric focusing (IEF) with conventional IEF systems. The equipment, which can be fixed on the cooling plate of a conventional IEF system, consists ofa base plate on which ffappable electrode holders are fastened. The handling is simple and needs only little time. Graphite rods are used as electrodes, thus avoiding the use of buffer strips Samples are applied with special applicator strips -permitting the analysis of up to 19 samples on a 50 X 40 mm polyacrylamide gel and up to 44 samples on a 100 X 70 mm gel. Only 30 min are needed for one IEF run.

πŸ“œ SIMILAR VOLUMES

A method for density gradient isoelectri
A method for density gradient isoelectric focusing in small scale
✍ Jostein Holtlund; Tom Kristensen πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 465 KB

A method for density gradient isoelectric focusing in small scale is described. It utilizes the same equipment as that ordinarily used for electrofocusing in polyacrylamide gels. thus allowing several samples to be analyzed in the same experiment and the simultaneous electrofocusing in density gradi

A new method for pH determination in iso
A new method for pH determination in isoelectric focusing experiments
✍ C. Secchi πŸ“‚ Article πŸ“… 1973 πŸ› Elsevier Science 🌐 English βš– 388 KB

A rapid and reproducible method for the pH measurementin the effluent from density gradient electrofocusing is described. By this procedure, after preparative isoelectric focusing, the detection of protein zones and pH measurement can be accomplished simultaneously, by serially coupling a uv flow ce