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A Microtiter Plate-Based High-Throughput DNA Purification Method

✍ Scribed by K. Wang; L. Gan; C. Boysen; L. Hood

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
498 KB
Volume
226
Category
Article
ISSN
0003-2697

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✦ Synopsis

A fast, reliable, inexpensive, and high-throughput method to purify DNA has been developed. It is based on DNA amplification by polymerase chain reaction utilizing a mini spin-column made from a 96 -well membrane-bottomed assay plate. With this method, (50 %) of the DNA is recovered routinely using Sephacryl(500 \mathrm{HR}) as filtration media. Purified DNA can then be used in various enzymatic manipulations, such as sequencing and hybridization. This provides an economical alternative protocol for routine large-scale purification of DNA templates for sequencing or other enzymatic manipulations. 1995 Academic Press, Inc.

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