A High-Throughput Plasmid DNA Preparation Method

✍ Scribed by G.M. Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 345 KB
• Volume: 223
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1542

✦ Synopsis

Genome projects require a high-throughput method for DNA template preparation, which traditional protocols can rarely provide. We introduce here a new protocol for plasmid and cosmid DNA preparation based on alkaline denaturation in a 96 -well format. The essential improvement made in this protocol on previous 96 well preparation includes the purification by Pro-Cipitate in 96 -well microfilters. The yield and quality of DNA are sufficient for restriction digestion, radioactive sequencing, and automated fluorescent sequencing. It is also significantly more cost-effective than several of the commercial mini-prep kits. : 1994 Academic Press. Inc.