Development of a high throughput protein a well-plate purification method for monoclonal antibodies
โ Scribed by Jennifer Hopp; Ross Pritchett; Maria Darlucio; Junfen Ma; Judy H. Chou
- Publisher
- American Institute of Chemical Engineers
- Year
- 2009
- Tongue
- English
- Weight
- 256 KB
- Volume
- 25
- Category
- Article
- ISSN
- 8756-7938
- DOI
- 10.1002/btpr.247
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## Abstract The use of Raman spectroscopy for the qualitative and quantitative analysis of dilute aqueous solutions is of interest to the biopharmaceutical manufacturing sector. However, the inherent weakness of the Raman effect, coupled with spectral variability due to spurious signals from sample
Protein solubility is a critical attribute in monoclonal antibody (mAb) formulation development as insolubility issues can negatively impact drug stability, activity, bioavailability, and immunogenicity. A high-throughput adaptation of an experimental method previously established in the literature