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A method of equilibrium microdialysis using polyacrylamide gel

✍ Scribed by V.P. Demushkin; N.M. Shalina

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
192 KB
Volume
105
Category
Article
ISSN
0003-2697

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✦ Synopsis

An equilibrium microdialysis method has been developed using polyacrylamide gel. Use of 20% sucrose solutions and methyl Cellosolve provides easy preparation of polyacrylamide gels. Dialysis is complete within 2-2.5 h at 20Β°C. The method can be used for studies of binding ligands to substances with molecular weight over 20,000.

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## Abstract A method for drying polyacrylamide slab gels of various size and gel composition (T 7.5–15 %, C 2.67 %) is described. The destained gels, containing glycerol, are overlayered with gelatin and dried. Transparent and flexible sheets are obtained which can be stored for extended periods an