A convenient method of casting polyacrylamide gels
β Scribed by H. Tsuyuki
- Publisher
- Elsevier Science
- Year
- 1963
- Tongue
- English
- Weight
- 169 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A method is described for analyzing the radioactivity of 3H-labeled RNA after separation by gel electrophoresis. The gels were soaked in 10% acetic acid and were sliced. The gel slices were dehydrated in alcohol, then saturated with toluene, and finally permeated with a toluene-based scintillation f
An equilibrium microdialysis method has been developed using polyacrylamide gel. Use of 20% sucrose solutions and methyl Cellosolve provides easy preparation of polyacrylamide gels. Dialysis is complete within 2-2.5 h at 20Β°C. The method can be used for studies of binding ligands to substances with
UV shadowing has the potential of being a convenient and sensitive method for the location and isolation of individual bands of uv-absorbing material from polyacrylamide gels. The technique's simplicity and its ability to examine many gels simulbaneously should prove to be quite valuable. Photograph