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A magnetic colloid system for isolation of rare cells from blood for FISH analysis

✍ Scribed by Bill Martin; Jenny Bruce; Nigel Smith; David Liu; Lindy Durrant

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 87 KB
Volume: 17
Category: Article
ISSN: 0197-3851
DOI: 10.1002/(sici)1097-0223(199711)17:11<1059::aid-pd196>3.0.co;2-9

No coin nor oath required. For personal study only.

✦ Synopsis

To improve cell recovery of trophoblast and nucleated red blood cells from maternal blood for diagnosis of chromosomal abnormalities, we have investigated the use of a magnetic sorting system that utilizes a ferrofluid. The main advantage of this system is that the beads used are small enough to allow visualization of chromosome-specific sequences by fluorescence in situ hybridization (FISH). The ferrofluid was validated using MAb340, a trophoblast-specific antibody, and anti-CD71, used to sort for nucleated red blood cells. Antigen-positive cells could be efficiently sorted from a 1000-fold excess of antigen-negative cells and easily stained by FISH. We are currently evaluating its use on maternal blood samples.

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