Quantitative FISH analysis and in vitro suspension cultures of erythroid cells from maternal peripheral blood for the isolation of fetal cells
✍ Scribed by Klaus Huber; Jürgen Bittner; Brigitte Worofka; Alexander Rosen; Erich Hafner; Karl Philipp; Kurt Bauer
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 162 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0197-3851
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The development of a non-invasive prenatal diagnostic test using fetal nucleated red blood cells (NRBCs) isolated from the maternal circulation is hampered by the low frequency of these cells in maternal blood, requiring extensive enrichment procedures before any analytical procedure can be performe
Fetal cells present in the maternal circulation are a potential source of fetal DNA that can be used for the development of a prenatal diagnostic test. Since their numbers are very low, ampli®cation of fetal cells has been discussed for a long time. So far, most studies have focused on culturing fet
A notable degree of research attention is being focused on the use of fetal cells enriched from the blood of pregnant women as a non-invasive means of prenatal diagnosis. By using magnetic activated cells sorting (MACS) and ¯uorescence in situ hybridization (FISH), we have examined the ef®cacy of en