## To the Editor: Fetal nucleated erythrocytes circulating in maternal blood are a potential source of fetal DNA for noninvasive prenatal genetic diagnoses. Nucleated red blood cells (NRBCs) have several advantages over fetal lymphocytes, granulocytes, or trophoblasts. They are nucleated, and thus
Enrichment and detection of fetal erythroid cells from maternal peripheral blood using liquid culture
β Scribed by Jin-Yeong Han; Young-Hee Lee; Sang-Dong Sin; Joo-In Park; In-Hoo Kim; Goo-Hwa Je; Griffin P. Rodgers
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 197 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0197-3851
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Iron loaded transferrin (holotransferrin) was used for enrichment of fetal cells from peripheral blood of pregnant women. Cord blood samples were used to evaluate enrichment efficacy of single and double MACS separations. Blood samples were obtained from 10 pregnant women prior to chorion villus sam
In this study we evaluated the performance of a system for the enrichment, identification and analysis of fetal cells in maternal peripheral blood. Blood samples were collected from women after chorionic villus sampling and enriched for the presence of nucleated erythrocytes using a three-step proc
The development of a non-invasive prenatal diagnostic test using fetal nucleated red blood cells (NRBCs) isolated from the maternal circulation is hampered by the low frequency of these cells in maternal blood, requiring extensive enrichment procedures before any analytical procedure can be performe