Choice of anticoagulant can influence the analysis using fluorescence in situ hybridization of fetal cells enriched from maternal blood
✍ Scribed by Verena Brombacher; Vivian Kiefer; Carolyn Troeger; Yvan Vial; Sabine Minderer; Wolfgang Holzgreve; Sinuhe Hahn
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 49 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0197-3851
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✦ Synopsis
A notable degree of research attention is being focused on the use of fetal cells enriched from the blood of pregnant women as a non-invasive means of prenatal diagnosis. By using magnetic activated cells sorting (MACS) and ¯uorescence in situ hybridization (FISH), we have examined the ef®cacy of enriched fetal cells in determining fetal sex. An unexpected ®nding of this investigation was that the sensitivity of this analysis was in¯uenced by the anticoagulant used to treat the maternal blood samples. As such, samples treated with heparin showed signi®cantly lower detection rates than samples chelated with EDTA.