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DEVELOPMENT OF A MODEL SYSTEM TO COMPARE CELL SEPARATION METHODS FOR THE ISOLATION OF FETAL CELLS FROM MATERNAL BLOOD

✍ Scribed by DIANA W. BIANCHI; KATHERINE W. KLINGER; THERESA J. VADNAIS; MARY ANN DEMARIA; ANTHONY P. SHUBER; JOEL SKOLETSKY; PAT MIDURA; MATTHEW DIRISO; CHRISTINE PELLETIER; MICHELLE GENOVA; MARLENA S. ERIKSON; JOHN M. WILLIAMS

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
648 KB
Volume
16
Category
Article
ISSN
0197-3851

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✦ Synopsis

Three major methods have been described for the isolation of fetal cells from maternal blood: fluorescenceactivated cell sorting (FACS), immunomagnetic beads, and magnetic-activated cell sorting (MACS). To date, no study has directly compared fetal cell recovery using each of these methods. Here we describe our system using a 'model' male fetal cell mixed into female peripheral blood mononuclear cells. Fetal cell yields and purities were assayed by a quantitative polymerase chain reaction (qPCR) using chromosomes Yand 7-specific sequences. Fetal cell recovery was investigated by selection of CD71' cells or depletion of CD45' cells. Our data demonstrated variation in fetal cell recovery for all methods tested, although CD71' selection by FACS gave the best and most consistent results.

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