The synthesis of multifunctional symmetric primary amines 4 and 8 and the covalent binding of 5'-0dimethoxytrityl-deoxynucleoside derivatives to their amino groups (compound 5 and 9) is described. Different strategies for dedimethoxytritylation including the use of strong acidic ion exchangers or pr
A Liquid-Phase Process Suitable for Large-Scale Synthesis of Phosphorothioate Oligonucleotides
β Scribed by Bonora, G. M.; Rossin, R.; Zaramella, S.; Cole, D. L.; Eleuteri, A.; Ravikumar, V. T.
- Book ID
- 126274711
- Publisher
- American Chemical Society
- Year
- 2000
- Tongue
- English
- Weight
- 84 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1083-6160
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π SIMILAR VOLUMES
The experimental conditions necessary for reducing the cost of large-scale solid-phase oligonucleotide synthesis by the phosphite hiester approac41 have been studied using bigbly loaded polystyrene resins.
Nucleoside derivatives coupled to a multifunctional highly symmetric primary amine 3 built the fundamental of a convenient method for large scale oligonucleotide synthesis in solution. The basic purification for the fast isolation of intermediates is obtained by gel permeation chromatography. Monome