✦ LIBER ✦

A highly sensitive method for the estimation of pyrimidine dimers in DNA by high-pressure liquid cation-exchange chromatography

✍ Scribed by Hans-Joachim Breter; Dieter Weinblum; Rudolf K. Zahn

Publisher: Elsevier Science
Year: 1974
Tongue: English
Weight: 273 KB
Volume: 61
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(74)90403-5

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✦ Synopsis

We have developed a rapid, highly sensitive method for the separation of thymine-dimers from thymine on a cation-exchange resin, which is capable of detecting one dimer out of 5 X 10" to 10" thymine molecules (depending on the specific activity of the used material) by the means of high-pressure liquid chromatograpk.

The assay has been very useful in DNA-hybridization studies using (TT)-containing DNA-strands and there is evidence that the method will be valuable for photoreactivation studies of uv-damaged DNA and for the enzymes involved in dimer-excision studies.

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