A simplified method for the determination of taxanes in yew needles by reversed-phase (C18) high pressure liquid chromatography

✍ Scribed by Erik L. M. van Rozendaal; Gerrit P. Lelyveld; Teris A. van Beek

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 155 KB
• Volume: 8
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(199711/12)8:6<286::aid-pca374>3.0.co;2-g

✦ Synopsis

An analytical procedure for the large-scale qualitative and quantitative screening of paclitaxel (Taxol ® ) and related neutral taxanes in yew needles has been developed. The needles were selectively extracted with ethanol:water:acetic acid (80:19:1) and the resulting extract was purified from chlorophyll, 3,5-dimethoxy phenol, taxine alkaloids and cinnamoyl taxanes, by means of small-scale partition chromatography on Extrelut ® . After concentration, the taxanes were determined by high pressure liquid chromatography on a C 18 column with acetonitrile:water as eluent and ultra violet detection at 227 and 280 nm. Cinnamic acid was used as an internal standard. Various aspects such as extraction efficiency, recovery, peak purity, detection limits, robustness and reproducibility of the method have been evaluated. The methods is very labour efficient with only 20 min of manpower input per sample.