A rapid and highly sensitive method for determination of picogram levels of norepinephrine and epinephrine in tissues by high-performance liquid chromatography

✍ Scribed by Yoshiki Yui; Yoshinori Itokawa; Chuichi Kawai

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 261 KB
• Volume: 108
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90687-9

✦ Synopsis

We report a new, rapid, and sensitive high-performance liquid chromatographic method for the regional catecholamine determination in tissues. Deproteinized specimens are directly injected onto the double column (the top anion-exchange column; CDR-20, and the bottom cation-exchange column; Zipax-SCX) for the separation of catecholamines with 0.07 N NaH,PO, buffer as the mobile phase. The separated catecholamines then enter a continuous flow system and the reagents for the trihydroxyindole method are sequentially added and finally the fluorescent products are dispatched to the highly sensitive spectrofluorophotometer. The lowest detection limit of this system was about 10 pg. Analytical recoveries from samples averaged almost 100%. Amount and response (peak height) were linearly related up to 1 ng. Within-run and day-to-day coefficient of variations were 1.0 and 1.6% for norepinephrine, 1.0 and 1.8% for epinephrine, respectively (n = 10). This rapid method will provide a great deal for the accurate regional brain and heart mapping of catecholamines.