A high-speed liquid chromatograph with a flow-spectrofluorimetric detector and the ultramicro-determination of aromatic compounds

✍ Scribed by Hiroyuki Hatano; Yukio Yamamoto; Muneo Saito; Etsuo Mochida; Shigeharu Watanabe

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 551 KB
• Volume: 83
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)97054-7

✦ Synopsis

A high-speed liquid chromatograph has been used with a new spectrofluorimetric detector, which is more sensitive than a normal ultraviolet absorption or spectrophotometric detector for fluorescent compounds. The spectrofluorimeter is equipped with double-beam optics and with a flow-cell, 3 ,ul in volume, and is used to record the emission and excitation spectra of the separnted components during the chromatographic separation by stopping the elution at peak maxima. This technique enables both qualitative identification and quantitative determination of the separated components.

Selective recording of chromatograms is possible by varying the wavelengths for emission and excitation.

The technique is demonstrated using mixtures of vitamins Bz, Bj and B6, and of naphthalene, anthracene, pyrene, benz(u)anthracene, benz(u)pyrene and benz(e)pyrene have been separated and identified.