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A high-performance liquid chromatographic method for the determination of polyphosphoinositides in brain

✍ Scribed by Toshihiko Nakamura; Youko Hatori; Kouji Yamada; Masuhiro Ikeda; Teruaki Yuzuriha

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
567 KB
Volume
179
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple high-performance liquid chromatographic method for the determination of endogenous phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) in brain has been developed. PIP and PIP2 were derivatized with 9-anthryldiazomethane to yield (9-anthryl)PIP and di(9-anthryl)-PIP2. The derivatives were separated on a reversed-phase column using isocratic elution and detected with a uv detector. The detection limits of PIP and PIP2 were 0.25 micrograms. The method with uv detection was sufficiently sensitive to measure the concentrations of PIP and PIP2 in rat brain. The levels of PIP and PIP2 were increased in developing rat brain and were decreased after 10 min of ischemia.

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