𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A high-performance liquid chromatographic method for the determination of dolichyl phosphates in tissues

✍ Scribed by Kouji Yamada; Shinya Abe; Takeshi Suzuki; Kouichi Katayama; Tadashi Sato

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
393 KB
Volume
156
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A sensitive high-performance liquid chromatographic method has been developed for the determination of dolichyl phosphate homologs in tissues. Dolichyl phosphates were methylated and derivatized with 3-(9-anthryl)-diazo-2-propene to yield methyl 3-(9-anthryl)-2-propenyl dolichyl phosphates. As an internal standard, 2.2-didecaprenylethyl phosphate was used. The derivatives were separated on a reversed-phase column using isocratic elution. and detected fluorometrically. The method with fluorescence detection was sufficiently sensitive to measure the concentration of dolichyl phosphate homologs in tissues. e 1986 Academic PXSS, IX.

πŸ“œ SIMILAR VOLUMES

High-performance liquid chromatographic
High-performance liquid chromatographic method for the determination of phytate
✍ Ernst Graf; Frederick R. Dintzis πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 409 KB

An improved method for the determination of phytate (myo-inositol 1,2,3,5/4,6 hexakis (dihydrogen phosphate)) in plants is described. Phytate is extracted with 0.5 N HCI, purified and concentrated on an AG l-X8 anion-exchange resin, taken to dryness in a vacuum desiccator, and analyzed by reverse-ph

High performance liquid chromatographic
High performance liquid chromatographic method for determination of canrenone in serum
✍ Besenfelder, E. ;Endele, R. πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 215 KB πŸ‘ 2 views

Spironolactone (I), a potassium sparing diuretic, is widely used in the treatment of edema. Metabolic degradation starts with rapid deacetylation, leading to the short lived a-thiol [l]. Subsequent elimination of hydrogen sulfide yields canrenone (II), which exists in enzymatic equilibrium with canr

A high-performance liquid chromatographi
A high-performance liquid chromatographic method for the determination of stobadin pharmacokinetics in serum
✍ Ladislav Ε oltΓ©s; ZoltΓ‘n KΓ‘llay; Ε tefan Bezek; Viera FedelesΗ’vΓ‘ πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 303 KB πŸ‘ 2 views

A high-performance liquid chromatographic method was developed to determine stobadin pharmacokinetics in dog and man. The relative bioavailability of stobadin dipalmitate compared with dihydrochloride was 46.4 per cent in dog. In man peak serum concentrations ranged from 12 to 289 ng ml-1 after a si