A graphic method for the determination and presentation of binding parameters in a complex system
β Scribed by Hannah E. Rosenthal
- Book ID
- 107710833
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 358 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0003-2697
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We offer a novel graphical method for determining the number of essential sites in enzymes that contain multiple binding sites for a ligand. This method is applicable both to monomeric enzymes containing multiple "unspecific" sites (for protons, metal ions, etc.) and to oligomeric enzymes containing
The theory and practice of a simple fluorimetric method for the estimation of ligand binding parameters in ligand-enzyme complexes is described. In this method, the concentration of a ligand-binding enzyme and the dissociation constant of a ligand-enzyme complex were estimated solely from the total