A semi-integrated method for the determination of enzyme kinetic parameters and graphical representation of the Michaelis-Menten equation
β Scribed by Ali Naqui; Britton Chance
- Book ID
- 107712053
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 373 KB
- Volume
- 141
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A graphical method of determining the Michaelis-Menten constant free of the external mass transfer resistance for a packed bed immobilized enzyme system was illustrated with examples from 3 different enzyme reactions. The intercept at the ordinate obtained by the straight line extrapolation of data
nonlinear regression program for the analysis of the effect of pH on enzyme activity has been developed for the IBM micro range and compatible machines. The program conforms the V and K,,, pH profiles to one of four commonly occurring kinetic schemes. By using multiple linear regression the program