A Simple Fluorimetric Method for the Estimation of Ligand Binding Parameters in Ligand-Enzyme Complexes

✍ Scribed by V. Leskovac; S. Trivic; M. Pantelic

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 224 KB
• Volume: 214
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1519

✦ Synopsis

The theory and practice of a simple fluorimetric method for the estimation of ligand binding parameters in ligand-enzyme complexes is described. In this method, the concentration of a ligand-binding enzyme and the dissociation constant of a ligand-enzyme complex were estimated solely from the total concentration of a ligand and the total fluorescence of the ligand in the absence and in the presence of enzyme.