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โœฆ   LIBER   โœฆ

A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast

โœ Scribed by Klebe, Robert J.; Harriss, June V.; Sharp, Z. Dave; Douglas, Michael G.

Book ID
121224698
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
724 KB
Volume
25
Category
Article
ISSN
0378-1119

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โœฆ Synopsis

Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10(6)-10(7) transformants/microgram DNA. Detailed analysis of the parameters involved in PEG-mediated transformation of E. coli reveals basic differences between the PEG and standard CaCl2 methods for transformation of E. coli. PEG-mediated transformation of yeast is far simpler than existing protoplast methods and is comparable in efficiency. The new methods described here for PEG-mediated genetic transformation may prove to be of general utility in performing genetic transformation in a wide variety of organisms.

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## Abstract In order to genetically modify microorganisms capable of producing polyhydroxyalkanoate (PHA) biopolymers, a simple and rapid method to prepare freshly plated __Pseudomonas__ cells for transformation via electroporation was developed. This method can be used to transfer both replicative