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A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast

โœ Scribed by Klebe, Robert J.; Harriss, June V.; Sharp, Z. Dave; Douglas, Michael G.


Book ID
121224697
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
724 KB
Volume
25
Category
Article
ISSN
0378-1119

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Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10(6)-10(7) transformants/microg

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