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Visualized investigation of yeast transformation induced with Li+ and polyethylene glycol

✍ Scribed by Ping Chen; Hui-Hui Liu; Ran Cui; Zhi-Ling Zhang; Dai-Wen Pang; Zhi-Xiong Xie; Hu-Zhi Zheng; Zhe-Xue Lu; Hua Tong


Book ID
116903192
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
750 KB
Volume
77
Category
Article
ISSN
0039-9140

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A general method for polyethylene-glycol
✍ Klebe, Robert J.; Harriss, June V.; Sharp, Z. Dave; Douglas, Michael G. πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 724 KB

Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10(6)-10(7) transformants/microg