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A highly efficient polyethylene glycol-mediated transformation method for mushrooms

✍ Scribed by Gang Li; Ruixue Li; Qiuyun Liu; Qiang Wang; Min Chen; Baojian Li

Book ID
109329176
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
143 KB
Volume
256
Category
Article
ISSN
0378-1097

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Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10(6)-10(7) transformants/microg