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A flow-injection chemiluminescent method for the evaluation of the antioxidant activity of 5′-nucleotides

✍ Scribed by Zhouping Wang; Huan Xu; Jia Qian; Jing Ye; Zhen Yang; Huachao Sun; Yonghui Shi

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
536 KB
Volume
25
Category
Article
ISSN
1522-7235

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✦ Synopsis

Abstract

In the present work, a simple chemiluminescence (CL) method coupled with flow‐injection analysis for the evaluation of antioxidant activity of 5′‐nucleotides (5′‐AMP, 5′‐CMP, 5′‐GMP, 5′‐UMP) was proposed. It is based on inhibition effect of the studied substances on CL emission of luminol–potassium ferricyanide–pyrogallol. Experiments were performed to evaluate the nature of the inhibition by 5′‐nucleotides of the CL reaction and their antioxidant activities. Based on the experimental results, it was observed that 5′‐nucleotides are available antioxidants that could effectively scavenge superoxide anion free radicals in a concentration‐dependent way. This will provide a basis for further development of the use of nucleotides. Copyright © 2009 John Wiley & Sons, Ltd.

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