A continuous spectrophotometric assay for alkaline phosphatase with glycerophosphate as substrate
โ Scribed by Alessandro Bianchi; Eugenio Giachetti; Paolo Vanni
- Book ID
- 116098287
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 324 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0165-022X
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A continuous spectrophotometric assay for glucose 6-phosphatase is described. The method uses glucose dehydrogenase and mutarotase as ancillary enzymes. Glucose 6-phosphatase activity is measured by following NADH formation at 340 nm. The method is linear, at least up to 38 mU in the test which corr