𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A sensitive, continuous spectrophotometric method for assaying α-glycerophosphate dehydrogenase: Activation by menadione

✍ Scribed by A. Garrib; W.C. McMurray

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
244 KB
Volume
139
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

By use of a more sensitive method than standard assays, it is demonstrated that alpha-glycerophosphate dehydrogenase activity can be monitored continuously in rat liver mitochondria; maximum activity was obtained by sonication in the presence of Triton X-100. Vitamin K3 (menadione) seems to enhance the activity of the enzyme. The assay in the presence of menadione is linear over a much greater mitochondrial concentration (up to 250 micrograms of protein in the reaction mixture).

📜 SIMILAR VOLUMES

A Continuous Spectrophotometric Assay fo
A Continuous Spectrophotometric Assay for Ureidoglycolase Activity with Lactate Dehydrogenase or Glyoxylate Reductase as Coupling Enzyme
✍ M. Pineda; P. Piedras; J. Cardenas 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 475 KB

A spectrophotometric assay for ureidoglycolase activity (both ureidoglycolate lyase and hydrolase), based on the reduction of glyoxylate to glycolate catalyzed by glyoxylate reductase or lactate dehydrogenase with the stoichiometric and continuous NADH oxidation, is described. The assay has been opt

Assay of Succinate Dehydrogenase Activit
Assay of Succinate Dehydrogenase Activity by a Colorimetric-Continuous Method Using Iodonitrotetrazolium Chloride as Electron Acceptor
✍ P. Munujos; J. Collcanti; F. Gonzalezsastre; F.J. Gella 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 294 KB

A spectrophotometric assay method for determining succinate dehydrogenase activity is described in which iodonitrotetrazolium chloride is used as a final electron acceptor. The enzyme activity is determined by measuring the formation of formazan due to the tetrazolium salt reduction. The assay is co