Episomes carrying limited regions of the chromosome where 5S RNA genes have previously been located are described. The DNA purified from each of these episomes contains one gene per molecule for each of the three ribosomal RNA species as shown by hybridization experiments.
5S-RNA genes of barley are located on the second chromosome
β Scribed by A. Kolchinsky; V. Kanazin; E. Yakovleva; A. Gazumyan; C. Kole; E. Ananiev
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 902 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0040-5752
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β¦ Synopsis
The genes coding for 5S RNA in barley were cloned, sequenced, and their cluster was assigned to chromosome 2 using wheat-barley chromosome addition lines. High-resolution gel-electrophoresis of DNA and subsequent hybridization revealed new details of the organization of 5S DNA both in wheat and barley. The in situ hybridization of the cloned 5S gene with triploid endosperm nuclei also suggests that these genes are located in a single locus.
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