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5S-RNA genes of barley are located on the second chromosome

✍ Scribed by A. Kolchinsky; V. Kanazin; E. Yakovleva; A. Gazumyan; C. Kole; E. Ananiev


Publisher
Springer
Year
1990
Tongue
English
Weight
902 KB
Volume
80
Category
Article
ISSN
0040-5752

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✦ Synopsis


The genes coding for 5S RNA in barley were cloned, sequenced, and their cluster was assigned to chromosome 2 using wheat-barley chromosome addition lines. High-resolution gel-electrophoresis of DNA and subsequent hybridization revealed new details of the organization of 5S DNA both in wheat and barley. The in situ hybridization of the cloned 5S gene with triploid endosperm nuclei also suggests that these genes are located in a single locus.


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