A 9.2 kb Pstl restriction fragment, repeated tandemly head-to-tail in the genome, contains the 5.8S, 16-18S, and 23-28S ribosomal RNA (rRNA) genes of Cephalosporium acremonium, a filamentous fungus used at the industrial scale for production of cephalosporin antibiotics. These rRNA genes were locate
Linkage of 5S RNA and 16S+23S RNA genes on the E. coli chromosome
β Scribed by Vola, C. ;Jarry, B. ;Rosset, R.
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 426 KB
- Volume
- 153
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Episomes carrying limited regions of the chromosome where 5S RNA genes have previously been located are described. The DNA purified from each of these episomes contains one gene per molecule for each of the three ribosomal RNA species as shown by hybridization experiments.
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The genes coding for 5S RNA in barley were cloned, sequenced, and their cluster was assigned to chromosome 2 using wheat-barley chromosome addition lines. High-resolution gel-electrophoresis of DNA and subsequent hybridization revealed new details of the organization of 5S DNA both in wheat and barl
The plastid 16S-23S intergenic spacer region in Conopholis americana, a totally heterotrophic angiosperm in the family Orobanchaceae, has undergone large deletions, including the entire tRNA(Ile) gene and all but small remnants of the tRNA(Ala) gene. The length of the region is less than 20% of that