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Organization of the 5.8S, 16–18S, and 23–28S ribosomal RNA genes ofCephalosporium acremonium

✍ Scribed by Lucinda G. Carr; Paul L. Skatrud; Thomas D. Ingolia; Stephen W. Queener

Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
844 KB
Volume
12
Category
Article
ISSN
0172-8083

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✦ Synopsis

A 9.2 kb Pstl restriction fragment, repeated tandemly head-to-tail in the genome, contains the 5.8S, 16-18S, and 23-28S ribosomal RNA (rRNA) genes of Cephalosporium acremonium, a filamentous fungus used at the industrial scale for production of cephalosporin antibiotics. These rRNA genes were located in Pst 1 digests of C acremonium genomic DNA using a hybridization probe that contained the 5.8S, 18S, and 25S rRNA genes from the yeast Saccharomyces cerevisiae. This probe was also used in screening a recombinant lambda library to identify phage carrying rRNA genes ofC. acrernonium. Yeast rRNA genes contained separately on individual a2P-labeled plasmids were used to demonstrate that a cloned 7.2 kb C. acremonium sequence, represented in the repeated 9.2 kb Pstl fragment, contained DNA from the C. acremonium 5.8S, 16-18S, and 23-28S rRNA genes. These genes were ordered with the 5.8S gene located between the 16-18S and 23-28S rRNA genes. The order of the 16-18S, 5.8S, and 23-28S rRNA genes observed in C acremonium is the same as that observed in rRNA repeats of many other lower eucaryotes, e.g.S. cerevisiae, Aspergillus nidulans, and Neurospora crassa.

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