Suggestive evidence that genes controlling invasion and metastasis of T-cell lymphomas are located on mouse chromosome 3
✍ Scribed by Luc Verschaeve; Dr. Hendrik Verschueren; Thierry Vandendriessche; Dominique van Hecke; Steven Verhaegen; Patrick de Baetselier
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 889 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Cell lines differing in their malignant potential have been derived from the murine BW5 I47 T-cell lymphosarcoma. To evaluate the involvement of chromosomal aberrations in tumor progression within this model, we have analyzed the karyotypes and the in vitro invasiveness of I 3 related nonmetastatic and metastatic variants. Giemsa banding revealed the presence of several marker chromosomes, one of which was of particular importance. Depending on the cell line, four variants of this marker I were found: Marker la corresponds t o two translocated chromosomes 3, marker Ib is a deleted la marker, marker Ic is a Ib translocated t o a small unidentified chromosome fragment, and marker Id is a further deleted Ib marker. The la and Id markers were characteristic for the noninvasive, nonmetastatic lines, whereas the Ib and Ic markers predominated in the invasive, metastatic variants. The results suggest that metastasis-enhancing genes are located between the D and F I band of mouse chromosome 3 and that metastasis-suppressing genes are located between the F I and H band of the same chromosome.