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5-bromodeoxyuridine enhancement of Inoue-Melnick virus synthesis in human colon carcinoma cell lines

✍ Scribed by Dr. Y. K. Inoue; Y. Nishibe; T. Kimura

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 359 KB
Volume: 31
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890310402

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✦ Synopsis

Abstract

Inoue‐Melnick virus (IMV) synthesis in naturally infected human colon carcinoma cell lines can be enhanced by 5‐bromodeoxyurdine (BUDR). The optimal conditions for BUDR enhancement differed between two cell lines, SW‐480 and DLD‐1, SW‐480 cells produced 100 times more infectious IMV than DLD‐1 cells by BUDR treatment. A clonal difference in IMV synthesis in SW‐480 cells was also observed. The highest titer of virus was found in the culture fluid of clone H (SW‐480/H) cells treated with BUDR, and a herpes‐like virus was detected by negativestaining electron microscopy of concentrates of the culture fluid. One nonproducer clone (SW‐480/B) was also activated to produce virus when treated with BUDR.

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