Enhancement of Inoue-Melnick virus synthesis by 5-bromodeoxyuridine in human meningioma (MG-1) cells

✍ Scribed by Y. Nishibe; Dr. Y. K. Inoue; T. Kimura; A. Hase

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 467 KB
• Volume: 27
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890270304

✦ Synopsis

The authors found remarkable enhancement of Inoue-Melnick virus (IMV) synthesis b y 5bromodeoxyuridine (BUDR) in human meningioma (MG-1) cells, a virus-producer line of type 1 IMV. Treatment with BUDR resulted in rapid and abundant synthesis of infectious IMV in MG-1 cells. The titer of the cell-associated virus in treated cells increased approximately 6.0 log1 0 compared with that in untreated cells. Immunofluorescent antibody tests revealed that IMV-associated late antigen was induced by BUDR in the cytoplasm of approximately 50% of treated cells. A clonal difference was also found in the enhancement of BUDR o n the IMV synthesis in MG-1 cells. The most remarkable enhancing effect of BUDR was observed in the clone C line, and a herpes-type virus was detected by negative-staining electron microscopy in the culture fluid of the clone C treated with BUDR.